Over-expression in embryonic neurons

Ryan Rountree rountree at cmgm.stanford.edu
Thu Jan 29 22:18:57 EST 1998


I'm trying to overexpress a protein throughout the early embryo (300
cells or hopefully earlier)  particularly  in the neurons.  Does anyone
know which of the two Fire vectors pPD49.78 (hsp16-2 promoter) or
pPD49.83 (hsp 16-41 promoter) would be best for this?  Are there any
references you know of that might describe this early expression more
clearly, or other vectors that might be better to try?  Thanks for your
help.





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