PCR amplification from phage plugs

Morris Maduro maduro at lifesci.ucsb.edu
Sat Jul 25 08:30:51 EST 1998


Hello,

Has anyone done PCR directly on lysates obtained from coring a lambda
plaque into SM buffer?  I'd like to remove inserts from the Okkema lambda
gt11 library using primers flanking the EcoRI site in the Bgal coding
region, and am having difficulty getting PCR products this way (i.e. by
taking 1 uL of supernatant from a lambda plaque).

I'd appreciate any comments or suggestions.

Regards,
Morris

Morris Maduro
Department of MCD Biology
Biological Sciences II
UC Santa Barbara
Santa Barbara, CA 93106
lab: (805) 893-8090
fax: (805) 893-2005





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