Longevity in C. elegans

daemon at net.bio.net daemon at net.bio.net
Tue Mar 10 11:03:35 EST 1998


>From The Worm Breeder's Gazette, 1998, v. 15, No. 2, p. 56.

          AN ATTEMPT TO SLOW AGING IN C. elegans.
        7. NO EFFECT OF SODIUM ADENOSINETRIPHOSPHATE
   Vladimir V. Bakaev, box 45, Novosibirsk, 630107, USSR

The purpose of this study was to investigate the  effect  of
different  concentrations of sodium adenosinetriphosphate in
water solutions on nematode life span.  In  this  experiment
sodium adenosinetriphosphate  was  used in following diluti-
ons: 1:10(3),  1:10(4),  1:10(5), 1:10(6) and 1:10(7). Three
adult animals (3 - 5 days old) were kept in microtitre wells
containing 0,75 ml of liquid medium (with E. coli and witho-
ut  sodium adenosinetriphosphate) during 4 hours,  then they
were discarded and newborn larvae were transferred  in  next
wells (with sodium adenosinetriphosphate in any concentrati-
on) every day (one worm in one well). This investigation was
carried  out  in temperature +21oC and in the darkness.  The
>obtained results are presented in the following table.

=C7=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E0=
=C4=C4=C4=C4=C4=E0=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C9
=C5    Concentration of   =C5     =C5      Longevity (days)      =C5
=C5        sodium         =C5     =DC=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=E0=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E1
=C5 adenosinetriphosphate =C5  n  =C5  mean+/-S.E. =C5   maximal   =C5
=DC=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E4=
=C4=C4=C4=C4=C4=E4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=E1
=C5       Control         =C5 12  =C5  20,8+/-1,7  =C5      27     =C5
=C5                       =C5     =C5              =C5             =C5
=C5       1:10(3)         =C5 12  =C5   5,6+/-0,2  =C5       6     =C5
=C5                       =C5     =C5              =C5             =C5
=C5       1:10(4)         =C5 12  =C5   6,1+/-0,2  =C5       8     =C5
=C5                       =C5     =C5              =C5             =C5
=C5       1:10(5)         =C5 12  =C5  10,7+/-1,2  =C5      20     =C5
=C5                       =C5     =C5              =C5             =C5
=C5       1:10(6)         =C5 12  =C5  20,7+/-1,3  =C5      27     =C5
=C5                       =C5     =C5              =C5             =C5
=C5       1:10(7)         =C5 12  =C5  20,9+/-1.8  =C5      26     =C5
=D1=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E2=
=C4=C4=C4=C4=C4=E2=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E2=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=D6

The nematodes in concentration of sodium  adenosinetriphosp-
hate 1:10(3) were unable to have progeny, then, the concent-
rations 1:10(3) and 1:10(4) were  toxic.  The  concentration
1:10(5) was slightly toxic (the differences between longevi-
ties in concentrations  1:10(3),  1:10(4),  1:10(5)  and  in
control  were  statistically  significant,  P < 0,001 in all
these cases).  The longevities in concentrations 1:10(6) and
1:10(7) and in control were similar (the differences between
longevities in concentrations 1:10(6)  and  1:10(7)  and  in
control were statistically not significant,  P > 0,05 in all
these cases).

Conclusion: If the water solution of  sodium  adenosinetrip-
hosphate  was  applied  to C.  elegans during the whole life
span in above described conditions,  it was not able to pro-
long the life span of these animals.

Acknowledgment: The  author  wishes to express his thanks to
CGC for providing C. elegans (Bristol, N2) and E. coli OP50.

+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++

>From The Worm Breeder's Gazette, 1998, v. 15, No. 2, p. 57.

            AN ATTEMPT TO SLOW AGING IN C. elegans.
             9. NO POSITIVE EFFECT OF EPITHALAMIN
   Vladimir V. Bakaev (e-mail: Vlad at bakaev.nsk.ru), Anatolii V.
Efremov,  Vladimir  N.  Anisimov*  P.O.  Box  45,  Novosibirsk,
630107,  Russia *N.N.Petrov  Research  Institute  of  Oncology,
St.Petersburg, 189646, Russia

There are  data on  the life  span prolonging  effect of pineal
petide  preparation  epithalamin  in  mice, rats and Drosophila
melanogaster  [1,2].
The purpose of this study was to investigate the effect of dif-
ferent concentrations of epithalamin in water solutions on  ne-
matode  life  span.  In this experiment epithalamin was used in
following  dilutions  :  1:10(5),  1:10(6),  1:10(7),  1:10(8),
1:10(9) and 1:10(10). Three adult animals (3 - 5 days old) were
kept in microtitre wells containing 0,75 ml  of  liquid  medium
(with  E.  coli  and without epithalamin) during 4 hours,  then
they were discarded and newborn larvae were transferred in next
wells  (with  epitalamin  in  any concentration) every day (one
worm in one well). This investigation was carried out in tempe-
rature  +21o  C  and in the darkness.  The obtained results are
presented in the following table.
                                                      Table I
=C7=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E0=
=C4=C4=C4=C4=C4=E0=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C9
=C5    Concentration of   =C5     =C5      Longevity (days)        =C5
=C5                       =C5     =DC=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=E0=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E1
=C5      epithalamin      =C5  n  =C5  mean+/-S.E. =C5     maximal   =C5
=DC=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E4=
=C4=C4=C4=C4=C4=E4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E1
=C5       Control         =C5 12  =C5  17,4+/-2,5  =C5       29      =C5
=C5        1:10(5)        =C5 12  =C5  12,8+/-2,1  =C5       27      =C5
=C5        1:10(6)        =C5 12  =C5  15,1+/-1,9  =C5       28      =C5
=C5        1:10(7)        =C5 12  =C5  17,1+/-2,2  =C5       28      =C5
=C5        1:10(8)        =C5 12  =C5  19,6+/-2,2  =C5       29      =C5
=C5        1:10(9)        =C5 12  =C5  18,8+/-2,0  =C5       31      =C5
=C5        1:10(10)       =C5 12  =C5  18,7+/-1,7  =C5       25      =C5
=D1=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E2=
=C4=C4=C4=C4=C4=E2=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E2=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=D6
                                                      Table II
=C7=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E0=
=C4=C4=C4=C4=C4=E0=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C9
=C5    Concentration of   =C5     =C5      Longevity (days)        =C5
=C5                       =C5     =DC=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=E0=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E1
=C5      epithalamin      =C5  n  =C5  mean+/-S.E. =C5     maximal   =C5
=DC=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E4=
=C4=C4=C4=C4=C4=E4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E1
=C5       Control         =C5 12  =C5  23,5+/-2,3  =C5       33      =C5
=C5        1:10(7,5)      =C5 12  =C5  16,8+/-2,5  =C5       34      =C5
=C5        1:10(8,0)      =C5 12  =C5  19,3+/-1,7  =C5       33      =C5
=C5        1:10(8,5)      =C5 12  =C5  13,7+/-1,4  =C5       21      =C5
=D1=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E2=
=C4=C4=C4=C4=C4=E2=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=E2=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=D6
Conclusion: If the water solution of epithalamin was applied to
>C. elegans during the whole life span in above described condi-
tions,  it was not able to prolong the life span of these  ani-
mals.
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=
=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4=C4-
1. Anisimov V.N., Khavinson V.Kh., Morozov V.G. Twenty years of
    study on effects of pineal peptide preparation: Epithalamin
    in experimental gerontology and oncology.
    Ann.N.Y.Acad. Sci., 1994, 719: 483-493.
2. Anisimov V.N., Mylnikov S.V., Oparina T.I., Khavinson V.Kh.
   Effect of melatonin and pinela peptide preparation epithala-
   min  on  life  span and free radical oxidation in Drosophila
   melanogaster. Mech. Ageing Dev., 1997, 97: 81-91.





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