nna at leicester.ac.uk
Sat Nov 14 15:49:18 EST 1998
We have just started trying to look at GFP expression in C.elegans but are
having trouble with autoflourescence in the gut. We have tried both wt GFP
& Clontech EGFP (red-shifted), using a BioRad confocal microscope & manual
UV microscopes, all set up for FITC fluorescence.
We would be very grateful for any suggestions/references as to how to get
around this problem. On the other hand, we would also consider alternative
marker genes - any suggestions?
Sorry for the naive questions - new to this field!
Dr Alan J. Cann PhD, Department of Microbiology & Immunology,
University of Leicester, P.O. Box 138, Medical Sciences Building,
University Road, Leicester LE1 9HN, UK.
More information about the Celegans