integration with UV rays / coinjection with oligos

Morris Maduro maduro at lifesci.ucsb.edu
Fri Oct 2 10:46:56 EST 1998


Hello,

I apologize if this has been discussed before.  I have had no success using
UV irradiation to obtain integrated lines (both rol-6D and
unc-119-rescued), although others here have had some luck.   I have
previously used gamma rays with good, consistent results, but as there are
no convenient X-ray or gamma sources at UCSB, UV is my only option now.

For those that have tried UV to obtain integrants, can you send me a reply
stating (a) which machine was used (e.g. Stratagene 'UV Stratalinker
1800'), (b) the energy (dose), and (c) the frequency of integrants per F1?
I would also like to know if anyone has tried using coinjection with
oligonucleotides as described by Craig Mello, and whether this was
successful.

Thanks in advance,
Morris

Morris Maduro
Department of MCD Biology
Biological Sciences II
UC Santa Barbara
Santa Barbara, CA 93106
lab: (805) 893-8090
fax: (805) 893-2005





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