I ask you...

Vladimir V. Bakaev vlad at bakaev.nsk.ru
Tue Oct 13 10:24:14 EST 1998





>From The Worm Breeder's Gazette, 1998, v. 15, No. 4, p. 46.

          AN ATTEMPT TO SLOW AGING IN C. elegans
10. THE SLIGHT POSITIVE EFFECT OF C. elegans LARVAE EXTRACT

         Vladimir V. Bakaev, Andrey P. Ivlev-Duntau
            Box 45, Novosibirsk, 630107, Russia
                 E-mail: Vlad at bakaev.nsk.ru

C. elegans larvae (1 - 2 days old) were collected from sepa-
rate stock,  frozen and after 12 hours or more time, thawed,
centrifuged  and  mixed  with  S  medium containing E.  coli
(1:1),  this mixture was used as a medium for worms in expe-
rimental group. The medium for control group was prepared by
mixing  S  medium  containing E.  coli with S medium without
E. coli  (1:1).  Three  adult  animals (3 - 5 days old) were
kept in microtitre wells containing 0,5 ml of liquid  medium
(with E.  coli and with or without larvae extract) during 12
hours,  then they were discarded  and  newborn  larvae  were
transferred  in next wells every day (one worm in one well).
Dilutions of larvae extract (1:10;  1:100 and  1:1000)  were
applied on third day.  This investigation was carried out in
temperature +24¦C and in the darkness.  The obtained results
are presented in the following table.

 ÇÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄàÄÄÄÄÄàÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÉ
 Å    Dilutions     Å     Å      Longevity (days)        Å
 Å    of larvae     Å     ÜÄÄÄÄÄÄÄÄÄÄÄÄÄÄàÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄá
 Å     extract      Å  n  Å  mean+/-S.E. Å     maximal   Å
 ÜÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄäÄÄÄÄÄäÄÄÄÄÄÄÄÄÄÄÄÄÄÄâÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄá
 Å    No dilution   Å 12  Å            toxic             Å
 Å    1:10          Å 12  Å  19,9+/-1,5  Å       25      Å
 Å    1:100         Å 12  Å  15,0+/-1,4  Å       27      Å
 Å    1:1000        Å 12  Å  14,6+/-1,5  Å       22      Å
 Å    Control       Å 12  Å  16,0+/-1,6  Å       20      Å
 ÑÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄâÄÄÄÄÄâÄÄÄÄÄÄÄÄÄÄÄÄÄÄâÄÄÄÄÄÄÄÄÄÄÄÄÄÄÄÖ

Conclusion: If the larvae extract was applied to C. elegans,
it  was  able  slightly to increase their mean longevity (by
24,4 per cent,  not significant) in dilution 1:10.  Probably
extract prepared from larvae C. elegans contains any factor,
which  can slightly stimulate longevity of adult worms,  but
this effect is obviously insufficient for noticeable prolon-
ging of longevity.

Acknowledgment: The  authors  wish  to express his thanks to
CGC for providing C. elegans (Bristol, N2) and E. coli OP50.





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