about b-galactosidase

Kim sun ja sun at geguri.kjist.ac.kr
Tue Oct 20 11:19:00 EST 1998


Dear Worm Breeders,

We have worked with putitive ECM protein. The other day, I found that
secreted b-galactosidase could not function so lacZ construct that
contain transmembrane stop signal was used. Is it true? I have used NLS
containing LacZ promoterless vector. but If I microinjected new
construct  containing N-terminal secretary signal sequence, what will be
the expression pattern. I really appreciate if I got your reply.





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