affi-gel

lei xu lxu at indiana.edu
Tue Sep 8 21:29:07 EST 1998


Hi, all,

Has anyone tried to use affi-gel 10 or 15 to purify antibodies? I want to
couple a recombinant protein with affi-gel 10 (Bio-Rad) and then use the
coupled agarose beads to purify the specific antibody from the antiserums.
Right now, I am having some problems with the efficiency of coupling and
yield of the antibodies. If you have some experience of doing this, please
let me know. Any help will be appreciated.



Lei






                          ///////////////////////////////
                                   Lei Xu
                               Jordan Hall A308
                               Department of Biology
                               Indiana University
                               Bloomington, IN 47405
                                Tel: 812-855-7271
                          ///////////////////////////////





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