dsRNA Soaking

gidi bishemer at mishkei.org.il
Mon Aug 16 08:54:08 EST 1999


Hi chris. My name is gidi shemer and I'm also after my first year in the
phd (I'm working in the technion, israel in beni podbilewicz lab). I
have'nt tryed the soaking method but if you want some details, I thing
you should write to Ron Eliss. In the last meeting he showed  some nice
staff about this method with the gene fem-1. As for the positive control,
you can use apx-1. You should get dead embryos arested at about 50 cells
stage with hyper pharyngal cells. If you want more details, e.mail me at
work (bishemer at tx.technion.ac.il). bye, gidi.

"CHRIS.J.MEE" wrote:

> As a first year PhD student who is currently working with dsRNA
> interference in C.elegans i was interested to see in some of the
> abstracts at the 1999 International Worm Meeting that a number of
> people were using dsRNA soaking as a method of RNAi instead of
> microinjection.
> I dont seem to be able to find anything further on the technique so i
> was hopeing that someone may be able to fill me in on how its done
> and how successful it is compared to microinjection of dsRNA.
> I would also like to know if anybody has a good gene to use as a
> positive control in dsRNA injections as the genes i am looking at
> have not been used before and so i have no idea if they give an
> obvious phenotype. It may be that my injection technique needs work
> so a positive control with a good phenotype would help.
>
> Any help would be great
> Chris Mee
> University of Nottingham
> plxcjm at nottingham.ac.uk
> ---





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