freezing C. elegans

Eugene Leitl eugene.leitl at lrz.uni-muenchen.de
Wed Jun 16 08:49:02 EST 1999


We're about to culture some C. elegans, specifically to select them
for freeze-tolerance.

What is your oppinion as to which freeze-hardy strain to use best?

Is the following freezing protocol still current?
http://www.dartmouth.edu/artsci/bio/ambros/protocols/MGH_protocols/koelle_prot/X
0006_freezing_worms.html

Is the following bulk culture protocol any good?
http://www.dartmouth.edu/artsci/bio/ambros/protocols/other/liquid_culture.html

TIA,

Eugene Leitl





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