integration with UV-irradiation

Sander van der Linden slinden at niob.knaw.nl
Mon Apr 17 10:31:29 EST 2000


Hi all,

A frequently asked question?
I like to use UV-irradiation for integration of extrachromosomal arrays.

I would be grateful if anyone could send me a reply with answers on
the following questions:

a) energy dose (µJ x 100) used with a Stratagene UV Stratalinker 1800.
   (or even better, what settings do you find work best for the
Stratagene Stratalinker?)
b) frequency of integrants per F1.

kind regards,

Sander van der Linden


Hubrecht Laboratory
Uppsalalaan 8
3584 CT  Utrecht
The Netherlands
slinden at niob.knaw.nl





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