F3 screen for roller/cosmid retention?

Erich Schwarz schwarz at cubsps.bio.columbia.edu
Mon Jan 17 18:31:21 EST 2000


    I seem to recall that somebody out there made a stable transformant
line with roller/cosmids having a 50% loss rate, mutagenized the line
with EMS, and then screened for F3 clones whose rate of retention had gone
up to 100% (because a homozygous lethal mutation was only surviving if
the cosmid was retained).

    Did this really happen and can somebody give me a reference for it?
I've searched elegans.swmed.edu without luck but do remember hearing at
least a rumor about this screen.  Thanks in advance for any solid
information.


--Erich Schwarz
  schwarz at cubsps.bio.columbia.edu
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