Cracking

Alex Green greenad at muohio.edu
Tue May 2 12:53:04 EST 2000


   I am having a fairly simple problem in that my nematode growth agar is
cracking.  I want to keep my C. elegans on the surface of the agar and
recently they have been burrowing into the agar.  I am using 3.5 cm plates
to culture my worms on.  Any suggestions to help me prevent cracking?  I
have already tried using high and low humidity conditions.  I also do not
think it has to do with the percent agar in the media because that has been
constant, even before the cracking.

Thanks a lot,
Alex Green





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