cooperation needed

John A. Glomset jglomset at u.washington.edu
Fri May 26 15:55:21 EST 2000


I am a postdoc. working in non-wrom background Lab. Recently I identyfied
and characterized a phophalipase homologe in C.elegans and it might also
exist in germline because it was first found in testis.
I meet a problem in doing  in situ hybridization with whole mount C.elegans.
most  time I do the in situ , the result is not very consistant.

To publish this paper soon, I am looking for  some people who is skill at in
situ hybridization techneque to cooperate with.

Any one who would like to help will be highly apprecitaed and be a auther of
this article.

Please reply to:
zhaobw at u.washington.edu




Juan <abrah023 at gold.tc.umn.edu> wrote in message
news:8ecuiu$ff7$1 at mercury.hgmp.mrc.ac.uk...
>  Hi, I am wondering if someone has performed RT-PCR on sigle worms using a
>  light cycler. If so, could you please provide with some tips/protocol. I
>  would eternally thank you for it.
>
>  Juan
>  "Rougvie Lab"
>  ---



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