Embryo protein Extraction

Yonatan Tzur yonatan at iname.com
Wed Nov 1 05:22:33 EST 2000


I am trying to extract total protein from C. elegans embryos.
I collect embryos from 4 5cm NGM plates, and treat them with bleach
solution.
After sonication in SDS Sample loading buffer I end up with less then 0.1
mg/ml protein.

Is there a known protocol for a successful extraction ?



Yonatan Tzur

     _____________________________________________________
                        Yonatan Tzur

                 mail to :yonatan at iname.com
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    "E pur si muove"
    Gallieo Gallilay


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