GFP antibodies

Liz Ryder ryder at WPI.EDU
Fri Dec 7 12:41:03 EST 2001


Hi --
         I am trying to figure out what cells my gene of interest is 
expressed in, using a GFP fusion.  In order to help figure out what 
cells I'm seeing expression in, I want to do a double-labeling 
experiment with my GFP fusion and an antibody stain (to LIN-26) as a 
marker.  I'm assuming GFP is killed by fixation; am I right?  And if 
so, does anyone have advice about what commercially available 
anti-GFP antibodies work well to stain embryos?
         Thanks for your help!
         Liz Ryder

Liz Ryder
Dept. Biology and Biotechnology
Worcester Polytechnic Institute
100 Institute Rd.
Worcester, MA  01609
TEL: 508-831-6011
FAX: 508-831-5936

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