GFP antibodies

Nobody nobody at hgmp.mrc.ac.uk
Mon Dec 10 05:10:13 EST 2001


Liz Ryder <ryder at wpi.edu> wrote:
>  In order to help figure out what cells I'm seeing expression in, I want
>  to do a double-labeling experiment with my GFP fusion and an antibody sta=
in
>  (to LIN-26) as a marker.  I'm assuming GFP is killed by fixation;

GFP survives paraformaldehyde fixation just fine.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 *=
/
/* D-97078 Wuerzburg, Germany   email: krasel at wpxx02.toxi.uni-wuerzburg.de *=
/
/* "Science is the game we play with God to find out what His rules are."  *=
/

---




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