Many eggs from liquid culture

Clegg, Eric D Dr USACEHR Eric.Clegg at DET.AMEDD.ARMY.MIL
Wed Dec 19 10:20:00 EST 2001


The Clegg Lab published an abstract in the latest Worm Breeder's 
Gazette describing a new axenic medium that allows development of L1s 
to gravid adults under axenic conditions in about 3 days. That 
abstract contains a link to our home page where the preparation of 
the CeHR medium is described.

We are using that system to synchronously produce large numbers of 
gravid adults for genomic and proteomic studies. As many as 500,000 
synchronized L1s have been produced at one time, and it would be 
possible to produce more. For this, we routinely maintain 
synchronized axenic liquid cultures in CeHR medium. When the culture 
reaches early gravid adult stage, the adults are bleached and the 
embryos placed into M9 and held overnight to hatch. The M9 prevents 
further development. The next day, the synchronized L1s are placed 
into CeHR and allowed to develop to the desired stage.

I hope this is helpful.
Eric Clegg




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