IPTG in agarose

Skinner, Steve stephen.skinner at agresearch.co.nz
Sun Feb 4 15:29:07 EST 2001


Hi there,

We need to grow nematodes on agar plates with dsRNA-producing bacteria and
induce them with IPTG.
Can anyone confirm for me that IPTG is stable enough to be mixed in with hot
agar? Otherwise I guess we just add to the cold plates and let it diffuse
into the gel.

Thanks,    Steve


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