Growing worms with autoclaved E. coli on plates

W.Tsang wtsang at gpu.srv.ualberta.ca
Fri Feb 16 14:16:55 EST 2001


Hi everyone,

Does anyone have experience with growing N2s on NGM plates seeded
with autoclaved E. coli? I had grown synchronized L1s under this
condition and these animals appeared to arrest at L3. Adding hemoglobin
onto the plate (a source of heme) didn't seem to help at all.

Thanks very much in advance.

William Tsang
-------------
Lemire lab
University of Alberta
Edmonton, Canada


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