Growing worms with autoclaved E. coli on plates

Creg Burns Darby cdarby at Stanford.EDU
Tue Feb 20 14:26:54 EST 2001


I tried this years ago and had the same result. I as able to get worms to
grow by killing E. coli with formaledyde, washing thoroughly, and putting
the dead cells onto NGM. We surmised that the worms need solid matter to
feed on, and that autoclaving lyses the E. coli and makes it inedible.

Creg Darby, PhD					  lab: 650-723-2671
Department of Microbiology and Immunology	  fax: 650-723-1837
Stanford University Medical School		cdarby at stanford.edu
299 Campus Drive, Room D033
Stanford, CA 94305-5124

wtsang at gpu.srv.ualberta.ca ("W.Tsang") writes:

>Does anyone have experience with growing N2s on NGM plates seeded
>with autoclaved E. coli? I had grown synchronized L1s under this
>condition and these animals appeared to arrest at L3. Adding hemoglobin
>onto the plate (a source of heme) didn't seem to help at all.

>Thanks very much in advance.

>William Tsang
>-------------
>Lemire lab
>University of Alberta
>Edmonton, Canada

-- 
Creg Darby, PhD					  lab: 650-723-2671
Department of Microbiology and Immunology	  fax: 650-723-1837
Stanford University Medical School		cdarby at stanford.edu
299 Campus Drive, Room D033
Stanford, CA 94305-5124







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