SHill at shot.com
Thu Feb 22 11:52:56 EST 2001
Can anyone tell me how big of a problem the cuticle is when trying to lyse
worms for RNA isolation? Are there any enzymes/chemicals that can lyse the
worms with minimal or no physical manipulation (ie agitation, using a
Has anyone tried to isolated RNA from dauers? Is it harder to disrupt the
duaer cuticle? In other words, is there a special protocol just for
isolating RNA from dauers?
Has anyone used Ambion's "RNAlater" with c.elegans? It is used to store
tissue/cell while stabilizing and protecting the RNA from RNases.
Finally, does anyone know how long RNA can be left in a guanidine extraction
solution such as Trizol?
Sorry for all the questions!!
More information about the Celegans