cuticle penetration

Hill, Stephanie SHill at shot.com
Thu Feb 22 11:52:56 EST 2001


Can anyone tell me how big of a problem the cuticle is when trying to lyse
worms for RNA isolation?  Are there any enzymes/chemicals that can lyse the
worms with minimal or no physical manipulation (ie agitation, using a
homogenizer).
Has anyone tried to isolated RNA from dauers? Is it harder to disrupt the
duaer cuticle? In other words, is there a special protocol just for
isolating RNA from dauers?

Has anyone used Ambion's "RNAlater" with c.elegans?  It is used to store
tissue/cell while stabilizing and protecting the RNA from RNases.

Finally, does anyone know how long RNA can be left in a guanidine extraction
solution such as Trizol?

Sorry for all the questions!!
Thanks!


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