expression of foreign cDNAs - intron requirement?

Colin Dolphin colin.dolphin at kcl.ac.uk
Tue Jul 17 12:12:08 EST 2001


I'd like to do some structure-function studies on a human protein
expressed in C.elegans via its cDNA. On the basis that both GFP/Lacz,
when used as reporters, exhibit enhanced expression when containing
several artificial introns would it be a good idea to do the same with
this cDNA prior to transformation? If so, is there a "rule of thumb" to
follow, eg. intron sequence, how many, where, etc? I guess the "easiest"
way to do this would be to insert at blunt-cutting sites but is there an
alternative procedure?

Colin

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