How to embed worms in parrafin?

Brian kraemerb at u.washington.edu
Tue Oct 30 13:17:44 EST 2001


Hi
I am interested in staining worms with some chemical stains normally used on
paraffin embedded 6 micron sections of formalin fixed mammalian tissue.  I
want to make paraffin sections containing worms so that I can process them
in a similar fashion.  I have tried typical worm whole mount techniques with
histochemistry in question and failed miserably.

I was wondering if there are any established methods for embedding and
sectioning a gob of worms in paraffin.  I recognize that it will be very
difficult to tell what is going on in any given worm using this approach,
but I think it will suit my purposes.  I just want to compare neurons in
wild type with those in different mutant strains.

Any suggestions or advice on how to accomplish this is appreciated.

Thanks,

Brian Kraemer

-- 
Brian Kraemer, PhD
Seattle VA Medical Center/University of Washington
Building 1, Room 711
1660 S. Columbian Way
Seattle, WA 98108
Phone: (206) 277-3275


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