Freezing in Liquid Nitrogen

Sean Jabbar s-jabbar at northwestern.edu
Mon Feb 11 22:08:01 EST 2002


Hi,

Is there anyone who can provide a detailed, working protocol for freezing C.
elegans in liquid nitrogen? We currently are freezing them at -70 C, but for
some reason they do not survive when placed in the liquid nitrogen using our
current protocol.

Thanks in advance,

Sean Jabbar

s-jabbar at northwestern.edu

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