Freezing in Liquid Nitrogen

Vera Lahl one at gmx.de
Wed Feb 13 17:30:27 EST 2002


In our lab we freeze C.elegans in liquid nitrogen using the method
described in: The nematode Caenorhabditis elegans, William B. Wood, Cold
Spring
Harbor Laboratory, 1988.
If you have problems getting a copy, poste again and I will translate my
german copy for you.
Greetings, Vera Lahl

"Sean Jabbar" schrieb in Nachricht ...
>Hi,
>
>Is there anyone who can provide a detailed, working protocol for freezing
C.
>elegans in liquid nitrogen? We currently are freezing them at -70 C, but
for
>some reason they do not survive when placed in the liquid nitrogen using
our
>current protocol.
>
>Thanks in advance,
>
>Sean Jabbar
>
>s-jabbar at northwestern.edu
>
>---
>




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