GFP antibody for IP & western

nishiwak at rtc.riken.go.jp nishiwak at rtc.riken.go.jp
Wed Feb 27 21:46:48 EST 2002


Hi,

Some days ago, I sent a question about GFP antibodies for IP and western.
I received only one reply directly answering the question from Morris Maduro.

>I was told by another laboratory here (that do not study C. elegans)
>that the Quantum 3E6 monoclonal worked very well, while Clontech's did not
>work at all.  In the Rothman lab, we only tried the Clontech reagent when
>attempting IPs from C. elegans embryos, and were not successful.  We
>actually haven't tried 3E6 on worms.

>Regards,
>Morris

For people interested in in situ staining, Raffi Aroian suggested to refer the
following subject.

>Newsgroups: bionet.celegans
>Subject: GFP surviving fixation; GFP antibodies
>Date: 7 Jan 2002 14:48:34 -0000
>Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
>From: nobody at hgmp.mrc.ac.uk (Nobody)
>Sender: owner-celegans at hgmp.mrc.ac.uk

Best regards,
Kiyoji
------------------------------------------------------------
Kiyoji Nishiwaki
RIKEN Center for Developmental Biology
2-2-3 Minatojima-minamimachi, Chuoku
Kobe 605-0074, Japan
TEL: +81-78-306-0105
FAX: +81-78-306-0101
E-Mail: nishiwak at rtc.riken.go.jp
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