PCR test for HT115/HT115(DE3)?

Marc Perry marc.perry at utoronto.ca
Fri Jan 11 12:29:45 EST 2002


Colin--

One classical test for lysogeny is to super-infect the bacterial 
host.  In this instance the phage lambda derivative DE3 carries the 
immunity region from bacteriophage 21.  Therefore plating an 
appropriate phage with lambda immunity should lead to plaques and 
plating one with imm21 should not (you could also test the imm21 
phage on a non-lysogen to verify that it is capable of forming 
plaques).  As for PCR, I suspect any sequence from the left arm of 
lambda should work nicely.  Likewise, DE3 contains an insert of T7 
gene 1 (the RNA polymerase) and presumably this would also be trivial 
to detect using your PCR idea.

--Marc


Marc D. Perry
Department of Molecular and Medical Genetics
Faculty of Medicine
University of Toronto
1 King's College Circle
Toronto, Ontario
CANADA  M5S 1A8

Tel.: (416) 978-0117
FAX: (416) 978-6885
marc.perry at utoronto.ca

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