in situ hybridisation

Paul McVeigh p.mcveigh at qub.ac.uk
Tue Nov 5 05:03:21 EST 2002


Hi,
    I'm trying to perform in situ hybridisation on larval and
adult C. elegans, but I have had no success with my protocol.  I
am using the method reported in Kimber et al. (2002) Int. J.
Parasitol. on Globodera pallida.  This protocol has also worked
on Panagrellus redivivus genes.

Is there any reason why this particular protocol would not work
in C. elegans but would work in the (theoretically) similar P.
redivivus?

Can anyone point me towards a protocol that works in adult worms?

Thanks,
Paul McVeigh.

----------------------
Paul McVeigh
Parasitology Research Group
Queens University Belfast
p.mcveigh at qub.ac.uk

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