worms adhering to petri-plate walls

Nobody nobody at hgmp.mrc.ac.uk
Tue Nov 19 04:13:07 EST 2002


     Well, only one thing I am doing in this case which is putting enough
bacteria  but not filling the plates by it. I seed the bacteria as a
small spot in the middle. In this case, if the worm is wild type from the
side of smelling the food, it will come back to the food soon. This will
not give it a chance to make a mistake of curiously climp up the very
near wall to its wall.
     If it will not work, you might use highly osmotic solution
surrounding the spot of the bacteria to force the worms avoiding crossing
this circle.
nasser
Abd ElNasser Elashry
Lehrstuhl F=FCr Entwicklungsbiologie
III. Zoologisches Institut-Entwicklungsbiologie
Universit=E4t G=F6ttingen
Humboldtallee 34A,
37073 G=F6ttingen
Germany

"Ivacic, Lynn" wrote:

>  Are there any treatments or plate conditions that would discourage or
>  inhibit the worms from traveling up the sides of the plates and
>  consequently sticking, and then dieing? When monitoring a small
>  population of young (30, synchronous) adult worms, as many as 10 will
>  explore and expire in this manner, even with plenty of E. coli in the
>  center of the plate.
>  Thanks,
>
>  Lynn Ivacic
>  Marshfield Clinic Research Foundation
>  1000 North Oak Ave.
>  Marshfield, WI 54449-5777
>  ---

---



More information about the Celegans mailing list