Soaking in dsRNA

Paul McVeigh p.mcveigh at qub.ac.uk
Wed Oct 16 10:25:41 EST 2002


Hi,
    Can anyone give me a good RNAi soaking protocol for
C. elegans? I have tried soaking with dsRNA in sterile H20
overnight (about 20 hours) at 20degC, but most of the worms had
died by the next morning (control worms were dead also).  My
plates were week old, and the worms seemed healthy before they
were soaked.

Thanks,
Paul McVeigh

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Paul McVeigh
Parasitology Research Group
Queens University Belfast
p.mcveigh at qub.ac.uk

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