Internal ribosomal entry sequence in C.elegans?
colin.dolphin at kcl.ac.uk
Thu Oct 24 07:45:46 EST 2002
Has anyone tried to tag a gene with an IRES? If it were recognised then
maybe it could be inserted as a cassette with GFP/LacZ into the 3'UTR
region of a cloned gene to generate a bi-cistronic transcript reporter
expression from the downstream cistron. That way GFP/LacZ would fold
independently of the tagged gene product but would still act as a
reporter of expression.
More information about the Celegans