good disruption method (nitrogen bomb?)

Edward Kipreos ekipreos at cb.uga.edu
Wed Feb 5 16:41:22 EST 2003


Dear C.e. community,

I would like to know if anyone has tried to use a nitrogen bomb (also 
known as a "cell disruption bomb") to lyse C. elegans. If anyone has 
tried to use one, either with positive or negative results, I would 
appreciate hearing about it. We would like to disrupt C. elegans for 
biochemical analysis (such as co-IP) and have been using sonication 
and a french press, but would like to use a method that disrupts 
larvae, adults, and eggs quicker. If anyone has a good method for 
disruption, we would appreciate hearing about that as well. If I get 
any useful insights, I will post the results on the C.e. bionet.

thanks,
Edward Kipreos

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Edward T. Kipreos
Department of Cellular Biology
University of Georgia
353 Biological Sciences Bldg.
1000  Cedar Street
Athens, GA  30602-2607
phone: (706) 542-3862
FAX: (706) 542-4271
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