Update/Thanks - "Brittle" OP50

Andrew Spencer aspencer at biochem.wisc.edu
Fri Jan 3 10:47:49 EST 2003

Thanks for all the suggestions on my new lab/brittle OP50 problem. 
Although I was using 50% of the classical KPO4 buffer, that didn't 
seem to be the problem.  When I used Eric Lambie's NGM Lite (see 
WBG), the OP50 went back to it's old, soft self.  I also made a batch 
of regular NGM w/ 1/10th the normal amount of Ca++ added, and those 
were fine as well.  I never made plates in the lab I was in 
previously, and I'm making my own here.  So just a rookie mistake, I 

Thanks again,


Andrew G. Spencer, Ph.D.
Department of Biochemistry
University of Wisconsin-Madison
ph: 608-262-0005 x3428
em: aspencer at biochem.wisc.edu


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