Localization of Phytochelatins in C. elegans

Christian Collin-Hansen Christian.Collin-Hansen at chem.ntnu.no
Thu Jul 10 04:05:27 EST 2003


>  Does anyone know where the PCs are located after binding the metals in
>  these organisms?? Do they remain in the cytosol?
>  ---

No, I don't know the answer to the localization question, but I have a
hunch based upon anatomy and other activities that the gene product
may lie in coelomocytes.  These float within the internal fluids of
the worm and are well known to scavenge for various other compounds.
And, their cytoplasm contains characteristic flattened clear
membranous organelles that look suspiciously like small vacuoles. Only
a select few cells in the whole animal contain these mini-vacuoles, of
which the coelomocytes are the most prominent.

See our online WormAtlas Handbook for some LM and TEM images of the
coelomocytes.

If someone else confirms my suspicion [or can disprove it], please let
me know.

Best regards,

David Hall

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Okay, thanks a lot for these answers, folks! Does anyone have anything
to add? I would be very grateful for every word, either posted here
directly or sent to my e-mail address.

Christian Collin-Hansen
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