Background fluorescence when using GFP reporter genes

Alastair Grant A.Grant at uea.ac.uk
Thu May 15 10:02:40 EST 2003


We are using a couple of nematode strains with GFP reporters for HSP
production.  These are strains obtained from CGC, not transformed in-house.

We are having problems with high background fluorescence in both wild type
(N2) worms and in the reporter gene strains kept in pleasant conditions.
Worms are being fed on OP50 and kept using standard procedures.

We would be grateful for any suggestions of what might be causing
autofluorescence, and how we might reduce it.

Alastair Grant

Centre for Ecology, Evolution and Conservation (CEEC),
School of Environmental Sciences,
University of East Anglia, Norwich, NR4 7TJ, UK
Phone 01603 592 537 Fax 01603 507 719 Mobile/Voicemail 07941579036
Home page: http://www.uea.ac.uk/~e130
CEEC web page: http://www.uea.ac.uk/ceec


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