RNAi

[George Joshua]

Dear colleagues,

I've used the Roche SP6/T7 transcription kit for RNAi and had variable
results. Suspect some toxicity.
What (if any) is the generally accepted best kit? Have heard Ambion?
Thanks,
Josh.

G.W.P.Joshua
London School of Hygiene and Tropical Medicine
Dept. Infectious and Tropical Diseases,
Keppel St.,
London WC1E 7HT.
Tel:     (+44) (0)20 7927 2028
FAX:    (+44) (0)20 7637 4314
E-mail:  g.joshua at lshtm.ac.uk

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