Native protein extraction

[Aurelio, Oscar]

Can anyone point our group to a protocol to extract NATIVE protein 
(i.e. no Tri-Reagent/Trizol phenol-solubilized protein) extraction from 
C. elegans? We obviously wish to avoid using proteinase K to lyse the 
worms. My colleague wishes to perform kinetic assays on an enzyme from 
worms. Thanks very much in advance for any help.



Oscar Aurelio, Ph.D.
Assistant Professor
CSU Fullerton
Dept. of Biological Sciences-MH282
800 N. State College Blvd.
Fullerton, CA 92834-6850


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