expression pattern of genes in operons

Nick Lyssenko nxl902 at psu.edu
Wed Feb 18 12:08:16 EST 2004


Dear All:

What is the current consensus on how to determine the expression 
pattern of
genes that reside in an operon in the second, third, etc position? One 
way to
approach this problem that seems to be reasonable is to take a genomic 
sequence
from the end of the nearest to the operon 5' gene to the beginning of 
the
nearest 3' gene and then replace the gene of interest with a GFP 
sequence. Is
this a viable strategy?
I am going through the literature to see what has been done, but it 
would be
nice to receive a bolus of concentrated knowledge on this subject.

Thank you very much for your help

Nicholas Lyssenko
*************************************************
American Heart Association Predoctoral Fellow
The Huck Institute for Life Sciences and
Department of Biochemistry and Molecular Biology
513 Wartik Lab
Penn State University
University Park, PA 16802
*************************************************

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