Tagging membrane proteins

Karla M. Knobel kmknobel at pharmacy.wisc.edu
Tue Jan 18 04:40:44 EST 2005


Hi,

I'd like to tag my favorite membrane associated protein (a sensory 
neuron ion-channel ) with something in addition to GFP.  Does anyone 
have any suggestions for which tags (ie, FLAG, MYC, HA) work best for 
membrane proteins that are expressed at low levels in very restricted 
patterns?  Ideally I would do immunocytochemistry to reveal the tag so 
I'd like to know which tags and antibodies perform best in fixed worms 
(what fixation protocol do you use?).

Thanks for your suggestions, Karla
-- 
Karla M. Knobel, Ph.D.

School of Pharmacy
University of Wisconsin
777 Highland Avenue
Madison, WI   53705-2222

LAB PHONE (608) 265-3251
FAX (608) 262-5345
EMAIL kmknobel at pharmacy.wisc.edu


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