[Celegans] using Ambion cDNA synthesis kits on whole worm lysates?
kculliso at mail.med.upenn.edu
kculliso at mail.med.upenn.edu
Thu Jul 21 14:30:41 EST 2005
my dear friends,
has anyone used either the "Cells to cDNA" (Cat. #1722) or the "Cells-to-Signal"
kit (Cat.#1724) (made by Ambion) on whole worm lysates. I'm looking for a more
efficient way to prepare cDNA (for semi-quantitative PCR) from various
developmental stages of C. elegans worm populations. I would greatly appreciate
any feedback...
-Kevin C.
University of Pennsylvania
School of Medicine
Philadelphia, PA 19104
kculliso at mail.med.upenn.edu
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