[Celegans] Re: Counting alive and dead worms

Daniel Muschiol danielmuschiol at web.de
Mon Apr 10 06:00:46 EST 2006


Dear Sarah,

there is a paper that might be helpful for your purpose:

Bird, A. F.. A method of distinguishing between living and dead
nematodes by enzymatically induced flourescence. Journal of Nematology
, 103-105. 1979

The author states that the technique is quick (results in 15 min).
Anyway, it makes the use of fluorescein diacetate (FDA) and
fluorescence necessary and I never tried it by myself.

Best, Daniel



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