[Celegans] sudan black staining

gsliwoski at mclean.harvard.edu gsliwoski at mclean.harvard.edu
Wed Jan 4 15:04:26 EST 2006


Hi, hope I'm posting this correctly.
Anyway, found a pretty straight-forward protocol for Sudan Black staining in:

“The C. elegans PTEN Homolog, DAF-18, Acts in the Insulin Receptor-like
Metabolic Signaling Pathway” by Ogg & Ruvkun. Molecular Cell, Vol 2 (Dec.
1998), page 887-893. Specific protocol on page 892.

I was getting dense staining in gravid adults with some minor variability.
I was just washing the worms off the plates into 15 mL tubes or picking
specific worms into 1.7 mL tubes , depending on how precise I wanted to
be, and using a pap-pen for slide visualization. I also washed the worms
4-5 times with M9 after the staining. Hope this helps.
--Gregory Sliwoski





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