[Celegans] M9 buffer

gsliwoski at mclean.harvard.edu gsliwoski at mclean.harvard.edu
Wed Jan 4 16:00:21 EST 2006


In response to:
>Hello, I'm trying to make M9 buffer, but there is a fine particulate after
>autoclaving. Has anybody had this problem before? I suspect the MgSO4 is the
>culprit, would adding it after autoclaving help? Thanks! ----------- Cara
>Neades Wellcome/CRC Institute Tennis Court Road Cambridge CB2 1QR United
>Kingdom

A couple months ago, we made M9 and it formed the particulate as
described. It turned out that we had accidentally used K2HPO4 instead of
KH2PO4. When we used the KH2PO4 correctly, we had no problems.
--Gregory Sliwoski




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