[Celegans] collagenase treatment for staining

gsliwoski at mclean.harvard.edu gsliwoski at mclean.harvard.edu
Thu Jan 12 17:03:52 EST 2006


Hi, I'm trying to compile a protocol for Indirect Immunofluorescence (GFP)
for GABA in c elegans. I'm under the impression that collagenase treatment
is required for this anti-sera. However, I'm not very familiar with this
type of treatment. On sigma, collagenase IV is varied, as well as somewhat
expensive. I was wondering if anyone had tips on the standards for
ordering collagenase IV, as well as any protocols for this type of
treatment.
Thanks,
--Gregory Sliwoski
gsliwoski at mclean.harvard.edu




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