[Celegans] pPD95.77 vectors lacking unc-54 3´-UTR

Antonio amirviz at upo.es
Wed Jun 14 12:05:32 EST 2006


Dear all

We are having what we suspect an unespecific intestine GFP  labelling  
when using pPD95.77 vector from Fire´s collection.
We have heard rumors that this unespecific signal might come from the  
unc-54 3´-UTR included in the vector.
We plan to remove this 3´-UTR and incorporate the 3´-UTR of our gene of  
interest.

Does anyone know of any pPD95.77 variant (or 75, 79, 81 depending on  
the frames) with the unc-54 3´-UTR removed and thus ready for inserting  
our own 3´-UTR?

Thank you very much in advance for your help
All the best
Antonio


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Antonio Miranda Vizuete, PhD.
Centro Andaluz de Biología del Desarrollo (CABD-CSIC)
Dpto. de Fisiología, Anatomía y Biología Celular
Universidad Pablo de Olavide
Carretera de Utrera Km. 1
41013 Sevilla
ESPAÑA (SPAIN)

Teléfono		+34 954 349381
Fax			+34 954 349376
email		amirviz at upo.es



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