[Celegans] Re :Celegans Digest, Vol 18, Issue 4

kisan jadav baburao via celegans%40net.bio.net (by kisanb1 At rediffmail.com)
Wed Nov 15 05:45:03 EST 2006


hi,i feel it is due to contamination of your plate by other bacteria\'s which prevent c.elegans to lay eggs,you can do one thing is you just bleach them regularly so you will get rid of problem. we had same problem few days back so we know regularly bleach them and we are doing fineok bye kisancelegans At oat.bio.indiana.edu wroteSend Celegans mailing list submissions tocelegans At net.bio.netTo subscribe or unsubscribe via the World Wide Web, visithttp://www.bio.net/biomail/listinfo/celegansor, via email, send a message with subject or body \'help\' tocelegans-request At net.bio.netYou can reach the person managing the list atcelegans-owner At net.bio.netWhen replying, please edit your Subject line so it is more specificthan \"Re: Contents of Celegans digest...\"Today\'s Topics:1. Simultaneous bagging on 15 plates, N2 and 3 other strains(Kathryn Hedges)----------------------------------------------------------------------Message: 1Date: Sun, 12 Nov 2006 05:49:27 -0800 (PST)From: Kathryn Hedges Subject: [Celegans] Simultaneous bagging on 15 plates, N2 and 3 otherstrainsTo: khedges1 At yahoo.comCc: celegans At magpie.bio.indiana.eduMessage-ID: Content-Type: text/plain; charset=iso-8859-1Last week, I set up fifteen 35-mm NGM/OP50 plates with small chunkscontaining my various strains of worms*. I incubated them at 20C, theproper temperature for all my strains**. Friday afternoon, all theplates seemed to be full of healthy adults, various larvae, and oodlesof eggs. Friday evening, they were all full of bagged adults, variouslarvae, and newly hatched L1s. So they must have stopped laying eggsquite some time earlier. Both my control and heat shock worms were bagging. My treatment wormswere heat-shocked for 2 hours at 33C on a heating block with a lid overit, while controls stayed in the 20C incubator. I started pickingcontrol worms for microscopy 5 hours after my pre-experiment check,followed by experimental worms about 7.5 hours after the initialchecking. The heat shock worms were a bit worse, which could reflectbeing examined later a

nd having a faster metabolism for a couple ofhours.What would cause them to suddenly fail to lay eggs? None of the plates were starved; depending on the population, most ofthe plates had at least adequate food and most had a substantial lawn.The worms were merrily chowing down on OP50 until they died from beingfull of larvae. I\'ve been raising N2s and CL2070s for about a year, andI\'ve never seen anything like this. (The other strains, CL2122 andCL2166, are new to my lab, but seem to have similar development to N2now that I\'ve switched to Difco agar and glass-distilled water. Theyhave GFP reporters for mtl-2 and gst-4.)**We had some trouble with the incubator Friday evening (power surgethat tripped a breaker, not discovered until a few hours later) butbagging takes more than a couple of hours, right?. Our power is pretty\"dirty\" here at a rural college, and whenever we have windy weather, weget power drops and surges. Could an incubator respond to that kind ofabuse in some way that would affect egg-laying? Heat spikes, coldspells, electromagnetic phenomena?*The plates were poured 10/31/06, seeded with OP50 on 11/1/06, andchunked from 5-day-old unsynchronized plates on 11/7/06. By 10/10/06,they had substantial but not overcrowded populations and adults hadbeen laying eggs prolifically.If anyone else has experienced sudden failure in egg laying, I\'d liketo hear from you. (Especially if you know how to prevent it from recurring.)Sincerely,Kathryn HedgesGraduate Student, BiologyHumboldt State University1 Harpst St., Arcata, CA 95521619-602-9839 cell------------------------------_______________________________________________Celegans mailing listCelegans At net.bio.nethttp://www.bio.net/biomail/listinfo/celegansEnd of Celegans Digest, Vol 18, Issue 4***************************************


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