[Celegans] microscope specifications for D-type neurons in L1 and L4

[Susana Santos]

Dear all,

I'm starting my work with C. elegans (namely with D-type neurons) and  I'm
interested to know what are the microscope specifications for visualisation
of this kind of neurons in L1 and L4. I've been reading a lot on the subject
and it seams like the best general approach is, undoubtedly, DIC.

Is there a possibility to identify these neurons (GFP-marked) with a
commercial microscope (Nikon-Eclipse TE2000U) operating with a 60x, 1.4 NA
oil immersion objective?

Looking forward to hear from you.

Best,

Susana Santos

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Susana Santos, PhD
ICFO-Institut de Ciencies Fotoniques
Mediterranean Technology Park
08860 Castelldefels (Barcelona)
Spain
Tel: +34  93 55 34 050
Fax: +34  93 55 34 000
Email: susana.santos At icfo.es
http://www.icfo.es