[Celegans] microinjecting chemical compounds

Angela Parrish parrish at salk.edu
Wed Sep 6 19:48:02 EST 2006


I'm relatively new to C. elegans and I am interested in introducing
small hydrophobic chemicals into the worm.  I was wondering if anyone
had any experience or suggestions for trying to microinject compounds
into worm tissues.  Are some tissues better than others?  If I inject
a high concentration into the gonad, is it possible some amount of the
compound would still be present in an adult worm, or should I try to
target specific cells in the adult?

Any input would be fantastic.

-- Angela Parrish
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
The Jack H. Skirball Center for Chemical Biology & Proteomics
The Salk Institute for Biological Studies
10010 N. Torrey Pines Road
La Jolla, CA 92037
(858) 453-4100 x1945

http://cbpl.salk.edu/~wang/

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~



More information about the Celegans mailing list