[Celegans] RE: Celegans Digest, Vol 23, Issue 2

gidi laptop 2 via celegans%40net.bio.net (by bishemer from email.unc.edu)
Thu Apr 5 13:51:22 EST 2007


Hi,
I tried using Acridine Orange and it worked quite nicely for me, but that
depends on your purpose. If you just put in the media (5ug/ml) it will be
accumulated only in apoptotic cells and if you use perforation it penetrates
all cells and intercalate in the DNA
Gidi

Dr. Gidi Shemer
Goldstein lab
Dep. of Biology
UNC- University of North Carolina at Chapel Hill
Phone: (919) 843-8576
Fax: (919) 9621625
 
Mailing address:
CB#3280, Coker Hall,
The University of North Carolina at Chapel Hill
Chapel Hill, NC  27599-3280, USA
 
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Subject: Celegans Digest, Vol 23, Issue 2

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Today's Topics:

   1. vital dyes (Hinman, Channing L.)


----------------------------------------------------------------------

Message: 1
Date: Wed, 4 Apr 2007 13:16:46 -0400
From: "Hinman, Channing L." <CHinman from UTNet.UToledo.Edu>
Subject: [Celegans] vital dyes
To: <celegans from magpie.bio.indiana.edu>
Message-ID:
	
<E087C887B3452C48A6BBF16A6ABFD4D401DB25F0 from MSG01CV00.utad.utoledo.edu>
Content-Type: text/plain;	charset="us-ascii"

I am interested in vital dyes other than trypan blue if you have come
across some I would like to get some information on use and
availability. Thanks 



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